Science & Technology Archives

How to avoid HPLC missing peaks?

You have prepared the samples, and you injected the first injection you are sitting waiting for your beautiful peaks to show up. You waited a lot, but nothing at all. Assuming you were supposed to have a peak after 15 minutes and you did not get it. Just wait 15 minutes more. If nothing appears, so there might be an issue. This simple guide will assist you to overcome the issue of hidden peaks while using HPLC (High-Performance Liquid Chromatography). If you are not able to solve the issue, you can contact the service provider to prevent more damages to your device. You can also inform me if you had similar or new situations and how you were able to solve them. Check if you injected the sample Well, believe, or not this might be the issue. You are using many samples, and you forgot to inject the sample or to turn on the auto-injector, and you run the device. Basically, there will no peaks because there is no sample at all. So just inject your sample, and this will solve the issue. Check if you injected the ‘Correct sample.’ I know this might be silly, but you forgot to mark the vial, and you injected a vial of deionized water instead of your sample. Absolutely, no peaks will be found, so make sure you mark the vials regularly. Improper injection volume When you are working with different devices and constantly changing injection volume you might put it ‘Zero’ or ‘.02 instead of 20’. So, you should check the injection volume through method windows [...]

Education Synergy

By Mohamed Abouzid|2021-03-23T09:09:17+00:00August 29th, 2014|Press release, Science & Technology|0 Comments

Long time ago, from the moment which the human started to build his civilization, he started to search for resources. Resources for feeding his clan, protecting them and establishment of his own community. It was clear from the begging that collecting these resources without the knowledge of using it would be less than worthless! so he started to learn and educate himself , not only that, he also started to teach the new generation of his community how , where and when they use these resources , all that was for an aim of creating sold community to continue and to survive , then to develop one level by other among all ages . Till now, this moment which I am writing this essay, the concept of learning and sharing the education is becoming understood and generally accepted for most of world countries and most of organizations which call for it. The development of European Union by exchanging European Union students, is best example for discussing my idea’s philosophy. In the world, right now everything is built on exchanges, which mean in all situation the double winner situation, as the country, person. Or society share what we have to gain what it don’t have. Science is like the sea, and whatever we gain from it, it will not be over, and the whole process of learning and education have to be generated and continuously stimulated all time, over less the time will pass without achieving any development. for such reasons , it will be impossible for one education system , ministry , college or university to [...]